1.一种附子LAMP组合检测引物,其特征在于,包括:Fusarium oxysporum的LAMP引物:正向外引物F3、反向外引物B3、正向内引物FIP、反向内引物BIP及正向环引物LF;具体为:F3:AGTTTCTGATTATTACCTCCTAGT;
B3:GCGTTCTCATATTTACAATTGTTG;
FIP:GCTATGACTTTGTAATCCTGAT‑GGTATTTTCAGCCATAATTGAAG;
BIP:CGGACCAAGTGTAGATCTTGC‑CCTAATAAACTACTTACCCCTGTT;
LF:TCAACCTGTACCCACTCCAC;
Fusarium solani的LAMP引物:正向外引物F3、反向外引物B3、正向内引物FIP、反向内引物BIP、正向环引物LF及反向环引物LB;具体为:F3:GGGCGACGTTGGACAAAG;
B3:GGTGATACCACGCTCACG;
FIP:CCGGTTAGCGTCTGTTGTGA‑CCCTGATCCCTGCACACA;
BIP:GGTCCAACAATAGGAAGCCG‑CAAGGACCCAGGCGTACT;
LF:CGCCAAGAGGGTTTGGTGTTTTT;
LB:TGAGCTCGGTAAGGGTTCC;
Mucor circinelloides的LAMP引物:正向外引物F3、反向外引物B3、正向内引物FIP、反向内引物BIP、正向环引物LF及反向环引物LB;具体为:F3:TAAGTCATTGATAAAGTCCAGTT;
B3:ACCGACCTTGGTGACATTAC;
FIP:GGGAATTCAGTAGGTCCCCAA‑ ATTGAGCATTCTATTCATCCGT;
BIP:GGTAAGTTTTTTCATGCAAATACAT‑ TTCGTGACCTAGAGATGACG;
LF:CATCAACAGTGTGCTATTGAAT;
LB:GTCATTTGGCGATATACTAAA;
Athelia rolfsii的LAMP引物:正向外引物F3、反向外引物B3、正向内引物FIP及反向内引物BIP;具体为:F3:GAATTGCAGAATCCAGTGAAT
B3:GGCATTTCTACATGTCCTACT
FIP:GACTCTCAAACAGGCATGCC‑CATCGAATCTTTGAACGCAC;
BIP:TTGTCAAGGCTTGGATGTGAG‑ATTTTAAAGAGAGCCAGTCAGAT。
2.一种附子LAMP组合检测试剂盒,其特征在于,该试剂盒中包含权利要求1所述的LAMP组合检测引物。
3.根据权利要求2所述的附子LAMP组合检测试剂盒,其特征在于,包含:Fusarium oxysporum的LAMP检测溶液:0.2 μM正向外引物F3,0.2 μM反向外引物B3,
1.6 μM正向内引物FIP,1.6 μM反向内引物BIP,0.8 μM正向环引物LF,1.4 mM dNTPs,1×LAMP Buffer,1×二甲酚橙,0.8 M甜菜碱,8 mM MgS04,0.32 U/μL Bst DNA polymerase;
Fusarium solani的LAMP检测溶液:0.2 μM正向外引物F3,0.2 μM反向外引物B3,1.6 μM正向内引物FIP,1.6 μM反向内引物BIP,0.8 μM正向环引物LF,0.8 μM反向环引物LB,1.4 mM dNTPs,1×LAMP Buffer,1×二甲酚橙,0.8 M甜菜碱,8 mM MgS04,0.32U/μL Bst DNA polymerase;
Mucor circinelloides的LAMP检测溶液:0.2 μM正向外引物F3,0.2 μM反向外引物B3,
1.6 μM正向内引物FIP,1.6 μM反向内引物BIP,1.6μM正向环引物LF,0.8μM反向环引物LB,
1.4mM dNTPs,1× LAMP Buffer,1×二甲酚橙,0.8M甜菜碱,8mM MgS04,0.32U/μL Bst DNA polymerase;
Athelia rolfsii的LAMP检测溶液:0.2 μM正向外引物F3,0.2 μM反向外引物B3,1.6 μM正向内引物FIP,1.6 μM反向内引物BIP,1.4 mM dNTPs,1× LAMP Buffer,1×二甲酚橙,
0.8 M甜菜碱,8 mM MgS04,0.32U/μL Bst DNA polymerase。