1.霍山石斛微卫星DNA分子标记,其特征在于:所述微卫星标记的标号分别为:Hs39、Hs40、Hs41、Hs42、Hs44、Hs47、Hs48、Hs50、Hs54、Hs56、Hs57;
其核苷酸序列分别为:
Hs39:
gatcagactt ccttccaact gccaaccacc gcctgacttt cacctgacca ccactcgata 60gtcaattgtc gttagatcac cgcttgtcca tcaaacttct gcccttaagc tatcggactt 120ccgcctgact gctgccagat ttctactcaa ctctccaaac tattataatt ccacaaccta 180taagttaaag gacgggtcaa cctttgtaat taactcacat ttagtgaaat cttttcatct 240aggagaggcc tcatggtttt tcccactatg ggttttccac ataaaaaaat ctgttgtttt 300acttctctct ctctctctct ctctctctct ctctctctct ctctctctct ctctcaaatt 360acatgtttgt atgttggata tccgtgtatt gctaagatga aaaatttaca cgctcaacca 420ttgcgaatat tctaacttgc aacaatgaca aaagtcaaac aaagtcaaac ataaaatgtg 480cctttcccta atagtaatca tataacaaca atgactcata atctttttac tattatggct 540ccaagaatat tgttctaaaa tattttcata atcaactaca tgcaagctgc acctcaccat 600Hs40:gatcagagtt caacgccagat tagaaaagtc cttgtagagg ccatcacccc ggcggagggc 60gcggcatccc cacaggagaa ggtcaggttt gacaagtaat gtcaaaagat gatgaaatta 120ttacgtgcaa gcacaggacg tcgtccagag gcgaattaga acgcgccacg ttaccattta 180ctatataaca gagtgatgtg aagcatcttg attcgcctct gaactgtccg gtaactgcac 240attataattt ctcccaaaga tgacatagga cgaggtgtgg aggcagagca gaaaggcatc 300agatagcatc atggagataa tggtggagga agaagaggat tagaggcata gggagagtag 360aggaggaata gaggtttctt gttgggggga atggatagat ttgtcattaa gattgaggcg 420atgttgtcag gcaaagactt tgtacagtca attatttttc cacaactcag cgtctatcat 480tatacttgta ccaccttcat tttgcaacat taaaaatgta ttctctctct ctctctctct 540ctctctctct ctctctctct catacgcatc tctcaaaatt cacatatact tagagagatg 600ttcaacctga catatttata caaatacaag tgttagaatg aattgaatac cacaaattcc 660agcaagtttg aagcagccac taactgattt tatt 694Hs41:gatcaacctc tggtatcact tcgagctcta ttgttcttcc attaatcttc ctcttcagat 60agtatgctac cagttcttca tctgtcggat gaaagcgaaa gccaggcggc agagttacag 120gcgccatttt tgtactgtta aatctcttat caatcctctt ttgacttttc tccctctaag 180ctctgcttca aaaaaaccat taaaactatc tcttgatttg aacatagtca gacagaaaat 240aacagaagga gagagagaga gcataccaaa tctcacataa gagagagaga gagagagaga 300gagagagaga gagagagaga gagagagaga gggcggcggt ttaagaacct ctgtagcttt 360ataaaggcaa gaaggttgct ttcctcgcaa tcgagccttt actctctctc tctactttgt 420tttgctttgg ctttatatcc aaatattctg agattattgt cctttccttc atgagccatt 480ggtccacgtg gatggacgcc att 503Hs42:gatcgccaca aaacatcagc cgcaaataca tgaagtatgg aagctcaacg agattccatc 60tatccatcaa ctaagcacac acagagagag agagagagag agagagagag agagagagag 120agagagacct cctgttggaa ctctcgttgg ccttgaatac tatgatgatg aaacttcttt 180attgccacac ttctgttcaa catttccccc ttatatacac atcccagtcc tccctgtcct 240atcataaagg catctgagaa accaaaggct gcagtttgca agtctgatag tggaaactcc 300ttgaaatcaa gagaatcgct tcctaagtca ataaacttat tgcatatttt ggattttctg 360cgatggttgc atctcaacct ttcaatctgc tgaacatact cgtctctctg atgttggatt 420ttctgatttt caagcttaag cgctgctata gaagacatgg ttgaatccaa ttcaacagtt 480gccttatctt tacgatgaaa catttcacga acacgaacat caaggatagc tacattttcc 540attgtacttt gaagttcttt catgcttttt tctatttggt tcactagctc gttctgcttc 600aacttggtaa ctactatgag ctcttcaagc tcttctct 638Hs44:gatcgggcta caacattact tggatttact acatttttgg atgtgttgac tactaactag 60ccttatcggc cccacctcga cgtttctctc tcaaacgaag caatggttcg ctcatataac 120tcccacttat ggcataaaac ctccccgccc tctctctctc tctctctctc tctctctctc 180tccattcgac tctcatgtag gaactgccca ctagcccctc ttgcttttct ccctcattcg 240catgcgagga gtgctcgact ctacctattc gttcgcatga ccttgtacat gagaccctag 300ccttgtcctc tctcgtttta ttgaccgtgc acccaagtaa cttagatgcg attgtccgct 360cacgtctccg tctcttcctc cacgtgtccc aaactcagct acgcatgatc cctaacctga 420aacccctcgt tctcttt 437Hs47:gatcacaaat gctgaaacat cctagcaaat tatacagttc atttcaattt gtgtggacaa 60gcaaaataac ttaatttttt tttccctcca agagtgggcc ggttgagaaa tcaagtagag 120gcaccaatag ctgcatccat ctctgtagag ttctttatca agttcttgct tgaaggcaac 180ccagcaacga cattcgcttc aatgccacat tcatttgtcc ctcttaaaat cttgaaatag 240ccatcctgaa gagaaaacat aaaaaatttt gatagacaag tgttaccaaa actaaaccat 300acacaaattt tgccaagtat tattttgaaa catacatcac cccaatttct attccactgg 360ttagcaagta gctgcaaaac aaatataaga gataacaaaa aaattgtatc agaattggat 420ttaatctgta aaatgtaaaa gaaatgtgtg tgtgtgtgtg tgtgagagag agagagagag 480agaactcaaa cccaataatc ttctccttgc tcactagtgc cccatccaat taacttaaca 540gcatgtccac ctaagacatc acctgtgata tatctgtaaa ctccagattt gtagtgggca 600aaatcctgtt tataagaaaa taaaaatcag gtgacctgct tgattatatt gtaggattca 660ttataataaa gtaaaattaa acaatgaaaa tttctatctt ttgc 704Hs48:atcagcaaga gaagaattat agatatatag ccgatataaa taaagctcat gttgggatgt 60tcaggagtag tccatttgtt gagcacctaa accaacttca atttcctttt gcactttttt 120atgaaccctt tatttggcat cctcaaggga aaggccgtaa tgatgataat gagaatccag 180actctgatgg ttaggagtct ttacatttct tcgcttgtat gtgtatgtgt gtgtgtgtgt 240gtgtgtgtgt gtgtgtgtgt gtttgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt 300gtgagagaga gagagagaga gagagagaga gagagagaga gagagagaga gagagagaga 360gagtgtgtgt gtgtgtgtgt gtgaagataa actgtataac ttctatgagc cacgccgact 420taataatcgc aacgattttg gctataacta tctcacgtca cccagcactg tagcggagcc 480aacattacaa ctctgaggtg ctagttcata ttccacagcc gcaatgccac cctgtcaagt 540gccacgatac tcaagtat 558Hs50:gagcccaagc aaacctcaag aactcctaaa tttagcaaat attcaacata accctaacca 60taggataaga ataactatta aatgcaaata atacaaagcc aaaagttcaa acagcccttc 120agattcaaat ctcctgctca tctctctctc tctctctctc tctctctctc tctctctctc 180tctctctctc tctctctgaa tgcatggacc ccaaccaaag gggggtatct gattggtttt 240gcccaggttt tcgacttttg tagtattagg tcggtccttt cgggatcgac caaaccagcc 300cctcctagac ctgtctaaca tactcccctc ccaaagaatc atcttgtcct caaggtaaaa 360ggacgaaaat tgatgcttca tcctctcata tgattcccaa gtaatatcat actcaagtag 420attttttcac ttaatcaaca cttcttacgt ctccctttcg tactcctacc aactctagtg 480gttccaaaat cacctctaga ttctctgtca acatcactga taactctaca attgaggtgt 540Hs54:gatcaaagtc tcattggcct tcccagccaa cagccactct tcttcaagct ccggcctccc 60gcatgctgat aatggtggac gttctctcca agggcaggga atcatgctat aaggtatcag 120ttcccagctt ttctctctct ctctcagtct ctctctctct ctctctctct ctctctctct 180ctccctctct ctctctctct ctctctctct ctctctctca tgatttctcg aatttcgatg 240gttaggctcg gaacgccttc tattcttgct tggagaagga ggcggcgacg aagcattatt 300aaagtaacaa tatcaacact ccgcctgcgg ccaaacctac cacaagcgcc tacgataaac 360tatttgacgg cgacagagac ggcggcgacg gcgacggtgg cgacggcggc gaacggggag 420aagcatggaa aggaattctt tctcccatag aacccgat 458Hs56:gatcagcaga agcaagaata aaagtcgaat gctttcattg cagccagctt ttccttgggg 60aagggggact taggaagaga aagagagaga gagaaagaga gagagagaga gagagagaga 120gagagagaac tggaaattga tgcatgagct caagtccgtt aactaagaag cattctgggg 180ctcatctaat cttatataac aatcaggttg gccatcatac attcaaactc agcaatctgc 240acataaaata tctgcagaag aagccacatt aaagcaacaa aatttagttt gaagccattg 300tcgtctgtgg ccagggatga attattaagt gcggagatca tacgtagaga atctggttta 360tctatgtgaa ccgtaaaaat gtagcaaact gcgttttagc tgttcaccac tacgctttta 420ctattcacaa agacaaatct gaatgattct ttgtatttga cctctgaacg taataatctc 480tcttgaccat gaccggtaga agactcaag 509Hs57:gatcatagtt actgtttacc acttacattc taagtgtgtc attctaccat tcctcttttg 60tctatttttt ctcaaaagca atcaactaat atcttcccca atcccatatc tcatgatgat 120ttaaagaact atatattaga ttcaagtcaa catcttatgc ttttccttga atttgcatgg 180agttctcata tttaaatatt gataagaatt atcattattc attgcaaaat attatctcat 240tattggggat atttttttat tgttctcttc atatgcctat atatgcttta tacaaaacag 300acaacaaatc tctctctctc tctctctctc tctctctctc tctctctctc tcaccttccc 360atagttgaac aataaaaaat tatctttatt tttttaatag gcaaacaggg atgtgacaag 420gatggagata ttttgttctc ttgttcatct gcttgaacca actactgcac aatctatgct 480taacagcaaa atgccccaaa aaagattcaa cctgccttgt gttcctgcaa ccggcgcatc 540t 541。
2.根据权利要求1所述的一种霍山石斛微卫星DNA分子标记,其特征在于:
所述的Hs39、Hs40、Hs41、Hs42、Hs44、Hs47、Hs48、Hs50、Hs54、Hs56、Hs57十一个位点的引物序列为:
Hs39-F CAC CGC TTG TCC ATC AAA CTT C
Hs39-R CGC AAT GGT TGA GCG TGT AAA T
Hs40-F AAG ATT GAG GCG ATG TTG T
Hs40-R ACT TGC TGG AAT TTG TGG T
Hs41-F CAG GCG GCA GAG TTA CAG
Hs41-R GCC AAA GCA AAA CAA AGT AGA G
Hs42-F TCC ATC TAT CCA TCA ACT AAG C
Hs42-R GAC GAG TAT GTT CAG CAG ATT G
Hs44-F ATG GCA TAA AAC CTC CCC G
Hs44-R GAG CGG ACA ATC GCA TCT AAG T
Hs47-F TGA AAT AGC CAT CCT GAA GAG
Hs47-R GGA GAA GAT TAT TGG GTT TGA GT
Hs48-F AGG GAA AGG CCG TAA TGA TGA T
Hs48-R AGA GTT GTA ATG TTG GCT CCG CTA
Hs50-F CCA AGC AAA CCT CAA GAA CTC
Hs50-R CGA AAG GAC CGA CCT AAT ACT
Hs54-F CAG CCA ACA GCC ACT CTT CTT C
Hs54-R CGC CGT CAA ATA GTT TAT CGT AG
Hs56-F GCA GAA GCA AGA ATA AAA GTC G
Hs56-R ATA AGA TTA GAT GAG CCC CAG A
Hs57-F CCT TGA ATT TGC ATG GAG TT
Hs57-R TTT TGG GGC ATT TTG CTG TT。